Conjugation inEscherichia coli
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منابع مشابه
Conjugation in Escherichia coli.
Boyer, Herbert (Yale University, New Haven, Conn.). Conjugation in Escherichia coli. J. Bacteriol. 91:1767-1772. 1966.-The sex factor of Escherichia coli K-12 was introduced into an E. coli B/r strain by circumventing the host-controlled modification and restriction incompatibilities known to exist between these closely related strains. The sexual properties of the constructed F(+) B strain and...
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We have constructed Escherichia coli-Streptomyces shuttle plasmids which are capable of conjugal transfer from E. coli to Streptomyces spp. These plasmids contained the pBR322 and pIJ101 origins of replication and the RK2 (IncP) origin of transfer. The transfer of plasmid was specifically dependent the presence of a 760-base-pair, cis-acting, oriT-containing fragment and on RP4 (IncP) functions...
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Hfr and F' Lac male strains of Escherichia coli were mated with purine-requiring females which had been starved for purine. These females formed mating pairs with the males. However, a mating in the absence of purine markedly reduced the yield of recombinants. Transfer of F' Lac or of lambda prophage also occurred infrequently. It was concluded that deoxyribonucleic acid transfer from male to f...
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INTRoDucTioN................................................................ 320 EXPERIMENTAL APPROACH...................................................... 321 MATERIALS AND METHODS..................................................... 321 Media................................................................... 321 Bacterial Strains............................................................ 32...
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ژورنال
عنوان ژورنال: Biochemistry and Molecular Biology Education
سال: 2007
ISSN: 1470-8175,1539-3429
DOI: 10.1002/bmb.113